Literature summary for 2.7.8.29 extracted from

Kuge, O.; Saito, K.; Nishijima, M.
Control of phosphatidylserine synthase II activity in Chinese hamster ovary cells (1999), J. Biol. Chem., 274, 23844-23849.

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Protein Variants

Protein Variants	Comment	Organism
R97K	in contrast to the PSS II wild-type transformant, the R97K transformant exhibits 4fold higher phosphatidylserine biosynthetic activity than that in CHO-K1 cells. The phosphatidylserine biosynthesis in the R97K transformant is not inhibited at all but elevated by the addition of phosphatidylserine	Cricetulus griseus

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphatidylserine	The phosphatidylserine synthesis in a CHO cell mutant which lacks PSS I, EC 2.7.8.8, but has normal PSS II activity, is almost completely inhibited by the addition of phosphatidylserine to the culture medium, like that in the wild-type CHO-K1 cells. The phosphatidylserine synthesis in a PSS II-overproducing stable transformant is reduced by 35% upon addition of phosphatidylserine. Residue Arg-97 is critical for the exogenous phosphatidylserine-mediated inhibition	Cricetulus griseus

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	O08888	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
CHO cell	-	Cricetulus griseus	-

General Information

General Information	Comment	Organism
physiological function	PSS II activity is inhibited by exogenous phosphatidylserine and overproduction of PSS II leads to the loss of normal control of PSS II activity by exogenous phosphatidylserine. PSS II-overproducing cells cultivated without exogenous phosphatidylserine exhibit a normal phosphatidylserine biosynthetic rate similar to that in CHO-K1 cells. Stable transformation of R97K mutant PSS II, leads to a 4fold higher phosphatidylserine biosynthetic rate	Cricetulus griseus

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Release 2023.1 (January 2023)